| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Prevotella amnii | CGTTCAGCTCTCAGGCTCTG | CGCCTTTACATCATGCTCACC | 60.46 | 59.67 | 292 | 132 |
100.00%
|
100.00%
|
| Primer 2 | Prevotella amnii | AGCAATAACCCTGCACAAGC | ATTGGCGTGTAGACGGAGC | 59.11 | 60.15 | 238 | 126 |
100.00%
|
100.00%
|
| Primer 3 | Prevotella amnii | AGCAATAACCCTGCACAAGC | TATTGGCGTGTAGACGGAGC | 59.11 | 59.90 | 239 | 126 |
100.00%
|
100.00%
|
| Primer 4 | Prevotella amnii | AGCAATAACCCTGCACAAGC | ATATTGGCGTGTAGACGGAGC | 59.11 | 60.27 | 240 | 120 |
100.00%
|
100.00%
|
| Primer 5 | Prevotella amnii | TCGTTCAGCTCTCAGGCTC | CGCCTTTACATCATGCTCACC | 59.11 | 59.67 | 293 | 116 |
100.00%
|
100.00%
|
| Primer 6 | Prevotella amnii | TGCCTTTCGTTGGATAGCTTC | GGGCTTGGCACTTAGAATGC | 58.64 | 59.54 | 259 | 112 |
100.00%
|
100.00%
|
| Primer 7 | Prevotella amnii | TCTTGCCTTTCGTTGGATAGC | GGGCTTGGCACTTAGAATGC | 58.64 | 59.54 | 262 | 112 |
100.00%
|
100.00%
|
| Primer 8 | Prevotella amnii | ATCTCCGCAGCTATGGCAC | AGTGCAACCACAGCTTGC | 59.93 | 58.88 | 174 | 112 |
100.00%
|
100.00%
|
| Primer 9 | Prevotella amnii | ATCGTTCAGCTCTCAGGCTC | CGCCTTTACATCATGCTCACC | 59.54 | 59.67 | 294 | 110 |
100.00%
|
100.00%
|
| Primer 10 | Prevotella amnii | ATCTCCGCAGCTATGGCAC | ACCACAGCTTGCAACAACC | 59.93 | 59.18 | 168 | 107 |
100.00%
|
100.00%
|